MicroRNA
MicroRNAs (miRNAs) are short RNA molecules 19 to 25 nucleotides in size that regulate post-transcriptional silencing of target genes. A single miRNA can target hundreds of mRNAs and influence the expression of many genes often involved in a functional interacting pathway. MiRNAs has been shown to be involved in the pathogenesis of many allergic diseases including asthma, eosinophilic esophagitis, allergic rhinitis and eczema. MiRNA can be isolated from cells, tissues and body fluids such as serum, plasma, tears or urine. MiRNA expression can be detected in both tissue samples as well as in cell-free biological fluids such as serum or plasma. Current methodologies used for detecting miRNAs include quantitative PCR (qPCR), in-situ hybridization, microarrays and RNA-sequencing. MicroRNAs mediate post-transcriptional gene silencing of target genes by targeting the 3′ untranslated region of mRNA, with the seed region in nucleotide in the 5′end of miRNA being the crucial sequence. MiRNAs have been shown to regulate multiple allergic diseases and represent an exciting area of research. Several miRNAs have been showed to target key pathogenic pathways involved in allergic inflammation and have the potential to be developed into novel therapeutic targets. The methods reviewed above will aid researchers who are beginning to explore the field of miRNA in allergic inflammation, and help to avoid potential pitfalls involved in miRNA research.
References
1.Lu TX, Rothenberg ME. J Allergy Clin Immunol. 2018;141(4):1202–1207.
References
1.Lu TX, Rothenberg ME. J Allergy Clin Immunol. 2018;141(4):1202–1207.
Chromatin/Epigenetic
MicroRNA
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LIN28 inhibitor LI71
产品货号 : M22370
cas no: 1357248-83-9
LIN28抑制剂LI71是一种有效的细胞渗透性LIN28抑制剂,可消除LIN28介导的寡尿苷化,IC50为7 uM。LIN28抑制剂LI71直接结合冷休克结构域(CSD),抑制白血病细胞中LIN28针对let-7的活性,胚胎干细胞。 -
miR-21 inhibitor 37
产品货号 : M17041
cas no: ——
miR-21 抑制剂 37 是一种新型 miR-21 功能抑制剂,在 HeLa-miR21-Luc 测定中 EC50 为 5.3 uM。 -
miR544-IN-1
产品货号 : M14844
cas no: 522606-67-3
miR544-IN-1 (SID 3712249) 是 miR-544 生物发生的选择性小分子抑制剂。 -
BzDANP
产品货号 : M13112
cas no: 1994258-88-6
BzDANP 是 pre-miR-29a 成熟的小分子调节剂。 -
myomiRs-IN-1
产品货号 : M11630
cas no: 1392224-59-7
myomiRs-IN-1 是一种小分子探针,可选择性抑制肌源性 microRNA (myomiRs),包括 miR-1、miR-133a 和 miR-206。