Sodium Oxamate

本公司产品仅用于科研实验，不得用于人体或动物的临床与诊断

草酸钠是一种 LDH 抑制剂。草酸钠 (SO) 通过下调 CDK1/细胞周期蛋白 B1 途径诱导 G2/M 细胞周期停滞，并通过增强线粒体 ROS 生成促进细胞凋亡。

Sodium Oxamate is an LDH inhibitor.?Sodium oxamate (SO) induces G2/M cell cycle arrest via downregulation of the CDK1/cyclin B1 pathway and promotes apoptosis through enhancement of mitochondrial ROS generation.It inhibits L(+)-lactate dehydrogenase and derails the entire gluconeogenic pathway.NETosis and lactate accumulation during LPS induced sepsis in mice was inhibited by sodium oxamate, LDH inhibitor.

Cell Proliferation Assay Cell Line:CNE-1 and CNE-2 cells Concentration:10 μM Incubation Time:24-72 hours Result:Showed IC50s of 74.6, 32.4 and 17.8 mM and 62.3, 44.5, 31.6 mM at 24, 48 and 72 h in the CNE-1 and CNE-2 cancer cells, respectively.Apoptosis Analysis Cell Line:NPC cells Concentration:0, 20, 50 and 100 mM Incubation Time:48 hours Result:Showed the increasement of early and late apoptotic cells in a dose-dependent manner. Increased the expression of pro-apoptotic Bax and cleaved-caspase-3, while reduced the anti-apoptotic signals of Bcl-2 and pro-caspase-3.Cell Cycle Analysis Cell Line:CNE-1 and CNE-2 cells Concentration:0, 20, 50 and 100 mM Incubation Time:24 hours Result:Showed a dose-dependent increase in the numbers of CNE-1 and CNE-2 cells in the G2/M phase.

Animal Model:Female Balb/c nude mice injected with CNE-1 cells Dosage:750 mg/kg Administration:Intraperitoneal injection; 750 mg/kg; once daily; 3 weeks Result:Inhibited the tumor growth when compared to either oxamate alone or irradiation alone.

SO, oxamate sodium, Aminooxoacetic acid sodium salt, Oxamic acid sodium salt

Apoptosis

Apoptosis

LDH|CDK|Apoptosis

——

——

565-73-1

111.03

C2H2NNaO3

>98% (HPLC)

In Vitro:?H2O : 12.5 mg/mL (112.58 mM)

[Na+].NC(=O)C([O-])=O

——

(-20℃)

With Ice Pack

≥ 2 years