Ecdysone

本公司产品仅用于科研实验，不得用于人体或动物的临床与诊断

蜕皮激素是昆虫和草药中的主要类固醇激素。蜕皮激素触发盐皮质激素受体激活并诱导细胞凋亡。通过蜕皮激素受体亚型 B1 的蜕皮激素信号传导是细胞自主死亡所需的。

Ecdysone is a major steroid hormone in insects and herbs. Ecdysone triggers mineralocorticoid receptor activation and induces cellular apoptosis. Ecdysone signaling through Ecdysone receptor isoform B1 is required cell autonomously for the muscle death.(In Vitro):Ecdysone (10, 100 nM) induced the expression of a-smooth muscle actin (SMA). Ecdysone elevated the expression of cleaved caspase 3 in a dose dependent manner. Ecdysone (10, 100 nM) suppressed cell motility and scratch wound closure to a comparable extent. Ecdysone (100 nM) induced a branched spindle mesenchymal-like cell shape. Ecdysone (100 nM) caused renal tubular inner medullary collecting duct cells apoptosis.(In Vivo):In male C57BL/6 mice, Ecdysone (6 μg/g/day; s.c.) significantly impaired renal function, and BUN levels were statistically significantly increased, amplifying the renal expression of α-SMA. Ecdysone also displayed MR-dependent nephrotic effects.

Ecdysone (α-Ecdysone; 100 nM; for 48 hours) causes renal tubular inner medullary collecting duct cells (IMCD) apoptosis. Ecdysone (10, 100 nM; for 48 hours) induces the expression of a-smooth muscle actin (SMA), a standard mesenchymal marker in a dose dependent fashion in inner medullary collecting duct cells (IMCD). Ecdysone elevates the expression of cleaved caspase 3 in a dose dependent fashion. Ecdysone (10, 100 nM; for 12, 24 hours) suppresses cell motility and scratch wound closure to a comparable extent. Ecdysone treatments (100 nM; for 24, 48 hours) induces a branched spindle mesenchymal-like cell shape. Apoptosis Analysis Cell Line:Inner medullary collecting duct cells (IMCD)Concentration:100 nM Incubation Time:For 48 hours Result:Caused renal tubular cell apoptosis.Western Blot Analysis Cell Line:IMCD cells Concentration:10, 100 nM Incubation Time:For 48 hours Result:Induced the expression of a-smooth muscle actin (SMA), a standard mesenchymal marker in a dose dependent fashion.

Ecdysone (α-Ecdysone; 6 μg/g/day; SC; for 14 days) evidently impaires kidney function marked by a statistically significant increase in BUN levels and amplifies renal expression of α-SMA in male C57BL/6 mice aged 10 weeks. Ecdysone confers an MR dependent nephropathic effect.

α-Ecdysone

Apoptosis

Apoptosis

Apoptosis

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3604-87-3

464.63

C27H44O6

>98% (HPLC)

In Vitro:?DMSO : 50 mg/mL (107.61 mM)

C[C@H]([C@H](O)CCC(C)(C)O)C1CC[C@@]2(O)C3=CC(=O)[C@@H]4C[C@@H](O)[C@@H](O)C[C@]4(C)C3CC[C@]12C

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(-20℃)

With Ice Pack

≥ 2 years