Apoptosis Activator 2

本公司产品仅用于科研实验，不得用于人体或动物的临床与诊断

一种细胞凋亡激活剂，可强烈诱导 caspase-3 激活、PARP 裂解和 DNA 片段化。

An apoptosis activator that strongly induces caspase-3 activation, PARP cleavage, and DNA fragmentation; leads to the destruction of cells (Apaf-1 dependent) with IC50 of 4 uM; exert cytostatic and cytotoxic effects on a variety of cancer cell lines while having little or no activity against the normal cell lines tested.

MDK83190 (Compound 2) (1-50 μM; 2 hours) effects against cell lines of lymphoid origin (CCRF-CEM, MOLT-4, and Jurkat) with IC50 values ranging from 4 to 9 μM.MDK83190 (Compound 2) (20 μM; 30 mins) activates procaspase-9 and procaspase-3 in a cyto c-dependent manner.MDK83190 (Compound 2) (0.1-100 μM; 22 hours)strongly induces caspase-3 activation, PARP cleavage, and DNA fragmentation, and finally killing cells with an IC50 of 4 μM. Cell Viability Assay Cell Line:Leukemia cell line: CCRF-CEM cells, MOLT-4 cells, and Jurkat cells Concentration:1-50 μMIncubation Time:2 hours Result:Was sensitive to compound 2-induced apoptosis.Western Blot Analysis Cell Line:Hela cellsConcentration:20 μM Incubation Time:30 mins Result:Induced procaspase-9 and procaspase-3 to active forms.Apoptosis Analysis Cell Line: Jurkat cells Concentration:0.1-100 μM Incubation Time:22 hours Result:Resulted inapoptosis and finally killed cells.

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Apoptosis

Apoptosis

Caspase-3

Cancer

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79183-19-0

306.1435

C15H9Cl2NO2

>98% (HPLC)

DMSO: ≥ 46 mg/mL

O=C1N(CC2=CC=C(Cl)C(Cl)=C2)C3=C(C=CC=C3)C1=O

1H-Indole-2,3-dione, 1-[(3,4-dichlorophenyl)methyl]-

(-20℃)

With Ice Pack

≥ 2 years