LAT1-IN-1

本公司产品仅用于科研实验，不得用于人体或动物的临床与诊断

LAT1-IN-1 (BCH) 显着抑制细胞对氨基酸的摄取和 mTOR 磷酸化，从而抑制癌症生长和细胞凋亡。

LAT1-IN-1 (BCH) ?significantly inhibit cellular uptake of amino acids and mTOR phosphorylation, which induces the suppression of cancer growth and apoptosis.?is a selective and competitive inhibitor of large neutral amino acid transporter 1 (LAT1).

BCH (1-100 mM; 3 days; KYSE30 and KYSE150 esophageal cancer cells) treatment suppresses cell proliferation in a dose-dependent manner.BCH (30 mM; 24 and 48 hours; KYSE30 and KYSE150 cells) treatment significantly increases cell population in the G0/G1 phase in both KYSE30 and KYSE150 cells, indicating that BCH induces cell cycle arrest at G1 phase. BCH (30 mM; 0-24 hours; KYSE30 and KYSE150 cells) treatment decreases phosphorylation of 4E-BP1 and p70S6K at 30 minutes and the decrease is continued for 24 hours. The amount of mTOR, 4E-BP1, and p70S6K proteins is slightly decreased. Cell Proliferation Assay Cell Line: KYSE30 and KYSE150 esophageal cancer cells Concentration:1 mM, 3 mM, 5 mM, 10 mM, 20 mM, 30 mM, 40 mM, 50 mM, or 100 mM Incubation Time:3 daysResult:Cell proliferation was suppressed in a dose-dependent manner. Cell Cycle Analysis Cell Line:KYSE30 and KYSE150 cells Concentration:30 mM Incubation Time:24 and 48 hours Result:Cell cycle arrest.Western Blot Analysis Cell Line:KYSE30 and KYSE150 cells Concentration:30 mM Incubation Time:0 hour, 0.5 hour, 1 hour, 3 hours, 6 hours, 24 hours Result: Phosphorylation of 4E-BP1 and p70S6K was decreased. The amount of mTOR, 4E-BP1, and p70S6K proteins was slightly decreased.

BCH (200 mg/kg; intravenous injection; daily; for 14 days; male BALB/c nude mice) treatment significantly delays tumor growth and decreases glucose metabolism, indicating that LAT1 inhibition potentially suppresses esophageal cancer growth in vivo. Animal Model:Male BALB/c nude mice (5-week-old) with KYSE150 cells Dosage:200 mg/kg Administration:Intravenous injection; daily; for 14 days Result:Significantly delayed tumor growth and decreased glucose metabolism.

BCH

Apoptosis

Apoptosis

LAT1|Apoptosis

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20448-79-7

155.19

C8H13NO2

>98% (HPLC)

H2O:12.5 mg/mL (80.55 mM; Need ultrasonic);DMSO:< 1 mg/mL (insoluble or slightly soluble)

C1CC2CC1CC2(C(=O)O)N

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(-20℃)

With Ice Pack

≥ 2 years