Mitophagy
Mitochondria are essential organelles for providing energy and metabolic intermediates. In addition, mitochondria are the main sites for reactive oxygen species (ROS) generation and play central roles in cell death. Mitophagy is a critical mechanism for bulk degradation of mitochondria through a process using macroautophagy machinery, and mitophagy mechanism holds a central role in mitochondrial quality control by recognizing damages to the mitochondria and selectively removing the damaged mitochondria. In this process, entire mitochondria are enclosed in a double-membrane vesicle, autophagosome, and delivered to lysosomes for hydrolytic degradation. Upon activation of mitophagy, Parkin ubiquitinates PARIS and promotes its degradation, thereby increasing transcription of PGC-1α and promoting mitochondrial biosynthesis. Dysregulation of mitochondrial function and accumulation of mitochondrial DNA mutations have frequently been observed in human cancers, the close relationship between mitophagy and cancer is evident through the regulation of expression of Parkin, BNIP3, NIX, and others by representative tumor suppressors such as p53 and Rb and oncogenes such as NF-κB, FOXO3, and HIF-1α. Parkin may act as a tumor suppressor. The expression of another important mitophagy regulator, PINK1, has been reported to be linked to the survival and prognosis of the adrenocortical tumor (ACT). Mitophagy plays a critical role to suppress cancer development. The increase of ROS could be responsible for facilitating cancer development upon mitophagy reduction.
References
1.Um JH,et al. BMB Rep. 2017;50(6):299–307.
References
1.Um JH,et al. BMB Rep. 2017;50(6):299–307.
Autophagy
Mitophagy
-
U0126
产品货号 : M27390
cas no: 109511-58-2
U0126 是一种有效的选择性 MAP 激酶非竞争性抑制剂,抑制 MEK-1 和 MEK-2,IC50 值分别为 0.07 和 0.06 μM。 U0126 抑制自噬和线粒体自噬。 -
Mtphagy Dye
产品货号 : M13406
cas no: 2137473-96-0
Mtphagy Dye 是一种用于可视化线粒体自噬的小分子荧光探针;具有合适的荧光特性,可在不损伤线粒体的长波长区域内检测线粒体自噬过程中的线粒体酸化。 -
P62-mediated mitophagy inducer
产品货号 : M12772
cas no: 1809031-84-2
P62 介导的线粒体自噬诱导剂 (PMI) 是一种小分子 p62 介导的线粒体自噬诱导剂,可在不招募 Parkin 或破坏膜电位的情况下激活线粒体自噬,并在缺乏功能齐全的 PINK1/Parkin 通路的细胞中保留活性; 1 uM 诱导 NQO1 活性加倍,并上调 Nrf2 和 ARE 依赖性抗氧化基因;增加自噬衔接分子 P62/SQSTM1 的表达和信号传导,迫使线粒体进入自噬;破坏 Nrf2-Keap1 相互作用。 -
Urolithin A
产品货号 : M10510
cas no: 1143-70-0
一种一流的天然化合物,口服后可在体外和体内诱导线粒体自噬;防止功能失调的线粒体随着年龄的增长而积累,延长秀丽隐杆线虫的寿命,提高与年龄相关的肌肉功能下降的小鼠模型的运动能力。