Imipramine

本公司产品仅用于科研实验，不得用于人体或动物的临床与诊断

Imipramine 是一种具有口服活性的叔胺类三环抗抑郁药。Imipramine 是一种具有抗肿瘤活性的 Fascin1 抑制剂。Imipramine 也抑制 5-羟色胺转运体 (serotonin transporter)，其 IC50 值为 32 nM。Imipramine 刺激 U-87MG 胶质瘤细胞自噬（autophagy），诱导 HL-60 细胞凋亡（apoptosis）。Imipramine 具有神经保护和免疫调节作用。

Imipramine is an orally active tertiary amine tricyclic antidepressant. Imipramine is a Fascin1 inhibitor with antitumor activities. Imipramine also inhibits serotonin transporter with an IC50 value of 32 nM. Imipramine stimulates U-87MG glioma cells autophagy and induces HL-60 cell apoptosis. Imipramine shows neuroprotective and immunomodulatory effects.

Imipramine (0.5-300 μM, 3 days) inhibits HCT-116 cell viability.Imipramine (20 μM) inhibits cell migration (7 h) and invasion (48 h).Imipramine (50 μM, 0-240 min) inhibites the PI3K/Akt/mTOR signaling pathway in U-87MG glioma cells.Imipramine (60 μM, 24 h) stimulates U-87MG glioma cells autophagy.Imipramine (80 μM, 24 h) induces HL-60 cell apoptosis.Cell Viability Assay Cell Line:DLD-1, HCT-116, and SW-480 Concentration: 0.5-300 μM Incubation Time:3 days Result:Inhibited cell viability and HCT-116 was more sensitive than DLD-1 and SW-480.Cell Migration Assay Cell Line:DLD-1, HCT-116, and SW-480 Concentration:20 μM Incubation Time:7?h Result:Produced a remarkable inhibition of migration in all assayed cell lines.Cell Invasion Assay Cell Line:HCT-116Concentration:20 μMIncubation Time:48?hResult:Inhibited cell invasion through Matrigel. Western Blot Analysis Cell Line:U-87MG Concentration:50 μM Incubation Time:0, 15, 30, 60, 120 and 240 min Result:Markedly inhibited the phosphorylation of both Akt (Ser473) and mTOR (Ser2481) in a time-dependent manner. Also dephosphorylated p70 S6K, a downstream target of mTOR.Cell Autophagy Assay Cell Line:U-87MG Concentration:60 μM Incubation Time:24 h Result:Stimulated the induction of autophagy through the redistribution of LC3 in U-87MG glioma cells.Apoptosis Analysis Cell Line:HL-60 Concentration:80 μM Incubation Time:24 h Result:Induced cell apoptosis.

Imipramine (20 mg/kg, i.p. or 15 mg/kg, p.o.; daily for 24 days) attenuates neuroinflammatory signaling and reverses stress-induced social avoidance in mice.Animal Model:Male C57BL/6 mice (6–8 weeks old) subjected to RSD (repeated social defeat) and HCC (home cage control)Dosage:20 mg/kg or 15 mg/kg Administration:Intraperitoneal injection or oral administration, daily for 24 days Result:Reversed RSD-induced social avoidance behavior, significantly increasing the interaction time, significantly decreased stress-induced mRNA levels for IL-6 in brain microglia.

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Apoptosis

Apoptosis

Apoptosis

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50-49-7

280.41

C19H24N2

>98% (HPLC)

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C=1C=CC2=C(C1)N(C=3C=CC=CC3CC2)CCCN(C)C

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(-20℃)

With Ice Pack

≥ 2 years