AFMK

本公司产品仅用于科研实验，不得用于人体或动物的临床与诊断

AFMK 是褪黑素的活性代谢物，具有抗氧化和自由基清除活性。 AFMK 是细胞凋亡调节剂，可提高吉西他滨在 PANC-1 细胞中的抗肿瘤作用。

AFMK is an active metabolite of Melatonin with antioxidant and free radical scavenging activity. AFMK is a modulator of apoptosis and improves the anti-tumor effect of Gemcitabine in PANC-1 cells.(In Vitro):In PANC-1 cell, AFMK in combination with Gemcitabine increases the inhibition of the production of HSP70 from 0.47 (Gemcitabine alone) to 0.13 (10 nM AFMK), 0.08 (0.1 nM AFMK) and 0.01 (0.001 nM AFMK). AFMK pretreatment significantly inhibits DNA damage and shows a very high hydroxyl radical scavenging potential with an IC50 of 338.08 nM.(In Vivo):In male C57BL mice, AFMK (10 mg/kg; i.p.) significantly reverses radiation-induced decline in the total antioxidant capacity of plasma. Pretreatment of AFMK shows a significantly lower value of comet tail length and % DNA in tail.

AFMK is one of the metabolites of melatonin and can be formed by both enzymatic or pseudoenzymatic and nonenzymatic metabolic pathways.AFMK pretreatment significantly inhibits DNA damage. AFMK shows a very high level of in vitro hydroxyl radical scavenging potential which was measured by an electron spin resonance (ESR) study. IC50 values resulting from ESR analysis was 338.08 nM. AFMK, a melatonin metabolite, is a sparingly investigated biogenic amine. AFMK administered to PANC-1 in combination with Gemcitabine inhibits the production of HSP70 and cIAP-2 as compared to the results obtained with Gemcitabine alone.Western Blot Analysis Cell Line:Human pancreatic carcinoma cell line (PANC-1)Concentration:0.001, 0.1, 10, 1000 nMIncubation Time:Result:Augmented the inhibitory effects on HSP70 expression from 0.47 (Gemcitabine alone) to 0.13 (10 nM AFMK), 0.08 (0.1 nM AFMK) and 0.01 (0.001 nM AFMK).

AFMK is a potent antioxidant in vivo. AFMK significantly reverses radiation-induced decline in the total antioxidant capacity of plasma in mice. Animal Model:Male C57BL mice 8 wk of age Dosage:10 mg/kg body weight Administration:Intraperitoneal injection Result:Radiation-induced decline in the total antioxidant capacity of plasma was significantly reversed in AFMK pretreated mice. AFMK-pretreated irradiated groups showed a significantly lower value of comet tail length and % DNA in tail.

Acetyl-N-formyl-5-methoxykynurenamine | Formyl-N-acetyl-5-methoxykynurenamine

Apoptosis

Apoptosis

Human Endogenous Metabolite|Antioxidant|Free radical scavengers|Apoptosis

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52450-38-1

264.28

C13H16N2O4

>98% (HPLC)

In Vitro:?DMSO : 50 mg/mL (189.19 mM)

O=CNC1=CC=C(OC)C=C1C(=O)CCNC(=O)C

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(-20℃)

With Ice Pack

≥ 2 years