GSK-3484862

本公司产品仅用于科研实验，不得用于人体或动物的临床与诊断

Gsk-3484862 是一种 DNA 甲基转移酶 DNMT1 的非共价抑制剂，具有抗癌活性。 Gsk-3484862 介导小鼠胚胎干细胞的整体去甲基化。

Gsk-3484862 is a non covalent inhibitor of DNA methyltransferase DNMT1 with anticancer activity. Gsk-3484862 mediates the global demethylation of mouse embryonic stem cells.

GSK-3484862 (0-10 μM, 6 or 14 days) induces dramatic DNA methylation loss.GSK-3484862 (0-10 μM, 4 days) results in a modest reduction in DNMT1 protein level. Cell Viability Assay Cell Line:Murine embryonic stem cells (mESC, wild-type (WT) or Dnmt1/3a/3b triple knockout (TKO))Concentration:2 μM and 10 μM Incubation Time:6 or 14 days Result:Induced dramatic DNA methylation loss, with global CpG methylation levels falling from near 70% in WT mESC to less than 18% after 6 days.Western Blot Analysis Cell Line:Murine embryonic stem cells (mESC, wild-type (WT) or Dnmt1/3a/3b triple knockout (TKO))Concentration:2 μM and 10 μM Incubation Time:4 days Result:Resulted in a modest reduction in DNMT1 protein level.

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Cell Cycle/DNA Damage

DNA/RNA Synthesis

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2170136-65-7

365.46

C19H19N5OS

>98% (HPLC)

In Vitro:?DMSO : 20.83 mg/mL (57.00 mM)

CCc1c(C#N)c(S[C@@H](C(N)=O)c2ccccc2)nc(N(C)C)c1C#N

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(-20℃)

With Ice Pack

≥ 2 years