Crotonoside

本公司产品仅用于科研实验，不得用于人体或动物的临床与诊断

巴豆苷在降低兔和猫的血压、降低兔、豚鼠和仓鼠离体肠条张力以及刺激豚鼠和仓鼠离体子宫方面比腺苷更有效。

Crotonoside has much more active than adenosine in reducing the blood pressure in rabbits and cats, in decreasing the tone of isolated intestinal strips of the rabbit, guinea pig and hamster and in stimulating the isolated uterus of guinea pigs and hamsters. Crotonoside inhibits the growth of S-18 and Ehrlich solid tumor in mice at the optimal doses of 96 mg/kg/day x 12 and 48 mg/kg/day x 12, with 1-T/C values of 65% and 6%, respectively.

Crotonoside (0-200 μM;) selectively inhibits the viability of AML cell line MV4-11, MOLM-13 (with FLT3-ITD mutant) and KG-1 (without FLT3-ITD mutant) in a dose-dependent manner with an IC50 of 11.6 μM, 12.7 μM and 17.2 μM, respectively.Crotonoside (0-100μM; 7 hours) inhibits the phosphorylation of FLT3 Erk1/2, Akt/mTOR and STAT5 is strongly inhibited by crotonoside at higher concentration of 12.5 μM in a concentration-dependent manner.Crotonoside (0-100 μM; 12 hours) exhibits a dose-dependent increase in the percentage of G0/G1 phase and a dose-dependent decrease in the percentage of G2/M and S phases cells. Crotonoside (0-100 μM; 24 hours) leads to concentration-dependent changes in the number of apoptotic MV4-11 cells, results in a dose-dependent decrease in the level of pro-caspase-3 and a dose-dependent increase in the level of the cleaved caspase-3 fragments. Cell Viability Assay Cell Line:AML cell line MV4-11, MOLM-13 and KG-1 cells Concentration:0-200 μM Incubation Time:72 hours Result:Inhibited AML cells growth than other cell lines tested.Western Blot Analysis Cell Line:MV4-11 cells Concentration:0 μM, 12.5 μM, 25 μM, 50 μM Incubation Time:7 hours Result:Inhibited AML cells growth than other cell lines tested.Cell Cycle Analysis Cell Line:MV4-11 cells Concentration:0 μM, 12.5 μM, 25 μM, 50 μM Incubation Time:12 hours Result:Induced cell cycle arrest in G0/G1.Apoptosis Analysis Cell Line:MV4-11 cells Concentration:0 μM, 12.5 μM, 25 μM, 50 μM Incubation Time:24 hours Result:Induced MV4-11 cell apoptosis.

Crotonoside(intraperitoneal and intravenous injection; 70 mg/kg, 35 mg/kg; once daily) induces a significant antitumor activity and inhibited xenograft tumor progress as compared to treatment with vehicle. Animal Model: NOD-SCID mice with MV4-11 cells Dosage:70 mg/kg, 35 mg/kg Administration:Intraperitoneal and intravenous injection; 70 mg/kg, 35 mg/kg; once daily Result:Produced significant AML tumor inhibition rates of 93.5% at 70 mg/kg.

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Others

Other Targets

Others

Cardiovascular Disease

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1818-71-9

283.24

C10H13N5O5

>98% (HPLC)

In Vitro:?DMSO : 25 mg/mL (88.26 mM)

C1=NC2=C(NC(=O)N=C2N1C3C(C(C(O3)CO)O)O)N

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(-20℃)

With Ice Pack

≥ 2 years