OSMI-1

本公司产品仅用于科研实验，不得用于人体或动物的临床与诊断

OSMI-1 抑制多种哺乳动物细胞系中的蛋白 O-连接 N-乙酰氨基葡萄糖 (O-GlcNAcylation)，而不定性改变细胞表面 N- 或 O-连接聚糖。

OSMI-1 inhibits protein O-linked N-acetylglucosamine (O-GlcNAcylation) in several mammalian cell lines without qualitatively altering cell surface N- or O-linked glycans. OSMI-1 is a cell-permeable O-GlcNAc transferase (OGT) inhibitor (IC50: 2.7 μM).

OSMI-1 (50 μM; 24 hours; CHO cells) treatment decreases the viability by about 50% after 24 hours.OSMI-1 (10-100 μM; 24 hours; CHO cells) treatment reduces global O-linked N-acetylglucosamine (O-GlcNAcylation) a dose-dependent manner. OSMI-1 inhibits OGT activity in cells. Cell Viability Assay Cell Line:CHO cells Concentration:50 μM Incubation Time:24 hours Result:Viability decreased by about 50% after 24 hours.Western Blot Analysis Cell Line:CHO cells Concentration:10 μM, 25 μM, 50 μM, 100 μM Incubation Time:24 hours Result:Reduced global OGlcNAcylation in a dose-dependent manner.

Mammalian and zebrafish toxicity profiles are strikingly similar, and zebrafish can usually serve as an intermediate step between cell-based evaluation and conventional animal testing. The zebrafish model is used to investigate OSM1-1 acute toxicity in vivo. The LC50 of OSM1-1 is 0.031?mg/mL (56?μM, 12?h) and 0.025?mg/mL (45?μM, 24?h) in zebrafish model.

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Others

Other Targets

O-GlcNAc transferase (OGT)

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1681056-61-0

563.64

C28H25N3O6S2

>98% (HPLC)

DMSO:83.33 mg/mL?(147.84 mM;?Need ultrasonic)

O=C(N(CC1=CC=CS1)CC2=CC=CO2)[C@H](NS(C3=CC(C=C4)=C(C=C3)NC4=O)(=O)=O)C5=CC=CC=C5OC

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(-20℃)

With Ice Pack

≥ 2 years