ONPG
CAS No. 369-07-3
ONPG ( 2-Nitrophenyl β-D-galactopyranoside )
产品货号. M27099 CAS No. 369-07-3
ONPG is a colorimetric and spectrophotometric substrate used to detect β-galactosidase activity.
纯度: >98% (HPLC)
COA
Datasheet
HNMR
HPLC
MSDS
Handing Instructions
规格 | 价格/人民币 | 库存 | 数量 |
1G | ¥267 | 有现货 |
|
生物学信息
-
产品名称ONPG
-
注意事项本公司产品仅用于科研实验,不得用于人体或动物的临床与诊断
-
产品简述ONPG is a colorimetric and spectrophotometric substrate used to detect β-galactosidase activity.
-
产品描述ONPG is a colorimetric and spectrophotometric substrate used to detect β-galactosidase activity.(In Vitro):To investigate the kinetic parameters of recombinant enzyme, the Michaelis-Menten constants (Km), turnover numbers (kcat), and catalytic efficiencies (kcat/Km) of Gal308 for ONPG and lactose are determined. The kcat and Km values are 464.7±7.8/s and 2.7±0.3 mM for ONPG, and 264.2±2.1/s and 7.1±0.8 mM for lactose, respectively. The kcat/Km value of the enzyme for ONPG (172.1 /s/mM) is 4.6-fold higher than that for lactose (37.2 /s/mM), which clearly demonstrated that the catalytic efficiency of Gal308 for ONPG is much higher than that for lactose. Gal308 displays high hydrolysis ability for ONPG (100%) and moderate activity for its natural substrate lactose (25.7%). However, the hydrolysis ability of the enzyme towards all other chromogenic nitrophenyl analogues is very weak, indicating that Gal308 is a β-galactosidase with narrow substrate specificity.
-
同义词2-Nitrophenyl β-D-galactopyranoside
-
通路Others
-
靶点Other Targets
-
受体——
-
研究领域——
-
适应症——
化学信息
-
CAS Number369-07-3
-
分子量301.3
-
分子式C12H15NO8
-
纯度>98% (HPLC)
-
溶解度——
-
SMILESOC[C@H]1O[C@@H](Oc2ccccc2[N+]([O-])=O)[C@H](O)[C@@H](O)[C@H]1O
-
化学全称——
运输与储存
-
储存条件(-20℃)
-
运输条件With Ice Pack
-
稳定性≥ 2 years
参考文献
1.Zhang Y, et al. Optimization of derivatization procedure and gas chromatography-mass spectrometry method for determination of bensulfuron-methyl herbicide residues in water. J Chromatogr B Analyt Technol Biomed Life Sci. 2015 Jul 15;995-996:31-7.
产品手册
关联产品
-
Pomalidomide-C2-NH2
Pomalidomide-C2-NH2 is a synthesized E3 ligase ligand-linker conjugate containing a Pomalidomide-based cereblon ligand and a linker.
-
Kinetensin
Kinetensin is a neurotensin-like peptide.
-
(3beta,7beta,12beta,...
(3β,7β,12β,20Z)-3,7,12-Trihydroxy-11,15,23-trioxo-lanost-8,20-dien-26-oic acid, a lanostane triterpenoid, demonstrates significant inhibitory effects on nitric oxide (NO) production in BV-2 microglia cells induced by n LPS, with an IC50 value of 9.55 uM.