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ONPG

CAS No. 369-07-3

ONPG ( 2-Nitrophenyl β-D-galactopyranoside )

产品货号. M27099 CAS No. 369-07-3

ONPG is a colorimetric and spectrophotometric substrate used to detect β-galactosidase activity.

纯度: >98% (HPLC)

COA Datasheet HNMR HPLC MSDS Handing Instructions
规格 价格/人民币 库存 数量
1G ¥267 有现货

生物学信息

  • 产品名称
    ONPG
  • 注意事项
    本公司产品仅用于科研实验,不得用于人体或动物的临床与诊断
  • 产品简述
    ONPG is a colorimetric and spectrophotometric substrate used to detect β-galactosidase activity.
  • 产品描述
    ONPG is a colorimetric and spectrophotometric substrate used to detect β-galactosidase activity.(In Vitro):To investigate the kinetic parameters of recombinant enzyme, the Michaelis-Menten constants (Km), turnover numbers (kcat), and catalytic efficiencies (kcat/Km) of Gal308 for ONPG and lactose are determined. The kcat and Km values are 464.7±7.8/s and 2.7±0.3 mM for ONPG, and 264.2±2.1/s and 7.1±0.8 mM for lactose, respectively. The kcat/Km value of the enzyme for ONPG (172.1 /s/mM) is 4.6-fold higher than that for lactose (37.2 /s/mM), which clearly demonstrated that the catalytic efficiency of Gal308 for ONPG is much higher than that for lactose. Gal308 displays high hydrolysis ability for ONPG (100%) and moderate activity for its natural substrate lactose (25.7%). However, the hydrolysis ability of the enzyme towards all other chromogenic nitrophenyl analogues is very weak, indicating that Gal308 is a β-galactosidase with narrow substrate specificity.
  • 同义词
    2-Nitrophenyl β-D-galactopyranoside
  • 通路
    Others
  • 靶点
    Other Targets
  • 受体
    ——
  • 研究领域
    ——
  • 适应症
    ——

化学信息

  • CAS Number
    369-07-3
  • 分子量
    301.3
  • 分子式
    C12H15NO8
  • 纯度
    >98% (HPLC)
  • 溶解度
    ——
  • SMILES
    OC[C@H]1O[C@@H](Oc2ccccc2[N+]([O-])=O)[C@H](O)[C@@H](O)[C@H]1O
  • 化学全称
    ——

运输与储存

  • 储存条件
    (-20℃)
  • 运输条件
    With Ice Pack
  • 稳定性
    ≥ 2 years

参考文献

1.Zhang Y, et al. Optimization of derivatization procedure and gas chromatography-mass spectrometry method for determination of bensulfuron-methyl herbicide residues in water. J Chromatogr B Analyt Technol Biomed Life Sci. 2015 Jul 15;995-996:31-7.
产品手册
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