ONPG
CAS No. 369-07-3
ONPG ( 2-Nitrophenyl β-D-galactopyranoside )
产品货号. M27099 CAS No. 369-07-3
ONPG is a colorimetric and spectrophotometric substrate used to detect β-galactosidase activity.
纯度: >98% (HPLC)
COA
Datasheet
HNMR
HPLC
MSDS
Handing Instructions
规格 | 价格/人民币 | 库存 | 数量 |
1G | ¥267 | 有现货 |
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生物学信息
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产品名称ONPG
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注意事项本公司产品仅用于科研实验,不得用于人体或动物的临床与诊断
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产品简述ONPG is a colorimetric and spectrophotometric substrate used to detect β-galactosidase activity.
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产品描述ONPG is a colorimetric and spectrophotometric substrate used to detect β-galactosidase activity.(In Vitro):To investigate the kinetic parameters of recombinant enzyme, the Michaelis-Menten constants (Km), turnover numbers (kcat), and catalytic efficiencies (kcat/Km) of Gal308 for ONPG and lactose are determined. The kcat and Km values are 464.7±7.8/s and 2.7±0.3 mM for ONPG, and 264.2±2.1/s and 7.1±0.8 mM for lactose, respectively. The kcat/Km value of the enzyme for ONPG (172.1 /s/mM) is 4.6-fold higher than that for lactose (37.2 /s/mM), which clearly demonstrated that the catalytic efficiency of Gal308 for ONPG is much higher than that for lactose. Gal308 displays high hydrolysis ability for ONPG (100%) and moderate activity for its natural substrate lactose (25.7%). However, the hydrolysis ability of the enzyme towards all other chromogenic nitrophenyl analogues is very weak, indicating that Gal308 is a β-galactosidase with narrow substrate specificity.
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同义词2-Nitrophenyl β-D-galactopyranoside
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通路Others
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靶点Other Targets
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受体——
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研究领域——
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适应症——
化学信息
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CAS Number369-07-3
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分子量301.3
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分子式C12H15NO8
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纯度>98% (HPLC)
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溶解度——
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SMILESOC[C@H]1O[C@@H](Oc2ccccc2[N+]([O-])=O)[C@H](O)[C@@H](O)[C@H]1O
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化学全称——
运输与储存
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储存条件(-20℃)
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运输条件With Ice Pack
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稳定性≥ 2 years
参考文献
1.Zhang Y, et al. Optimization of derivatization procedure and gas chromatography-mass spectrometry method for determination of bensulfuron-methyl herbicide residues in water. J Chromatogr B Analyt Technol Biomed Life Sci. 2015 Jul 15;995-996:31-7.
产品手册
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