Aflatoxin B1

本公司产品仅用于科研实验，不得用于人体或动物的临床与诊断

Aflatoxin B1 (AFB1) 是一类 1A 级致癌物质，是 Aspergillus flavus 和 A. parasiticus 的次生代谢产物。Aflatoxin B1 (AFB1)主要通过诱导抑癌基因 p53 中 codon 249 第三位点的 G-->T，从而引起突变。

Aflatoxin B1 (AFB1) is a Group 1A carcinogen and a secondary metabolite produced by Aspergillus flavus and A. parasiticus. Aflatoxin B1 (AFB1) primarily induces mutations by causing a G to T transversion at the third nucleotide of codon 249 in the tumor suppressor gene p53. In the body, Aflatoxin B1 (AFB1) can cause damage to the intestines and kidneys and has some teratogenic effects.

Aflatoxin B1 (0.5 mg/kg; i.g.; 每天 1 次连续 28 天) 对小鼠的肾组织造成明显的损伤。Aflatoxin B1 (0-0.1 mg/kg; i.g.; 在妊娠 6-18 天给药) 对新西兰白兔有致畸作用。Animal Model:35-day subacute toxicity CD-1 mice model Dosage:0.5 mg/kg Administration:i.g.Result:Caused edema and cell proliferation in some parts, occasionally severe inflammatory cell infiltration and bleeding.Animal Model:New Zealand White female rabbits Dosage:0.025, 0.05 and 0.1 mg/kg Administration:i.g.; give during days 6-18 of pregnancy Result:At doses of 0.025, 0.05 and 0.1 mg/kg, the incidence of wrist droop and eye socket enlargement in white rabbits was 3.57%, 4.00% and 14.28% respectively.And at a dose of 0.1 mg/kg, a few rabbits developed microphthalmia and heart defects.

Aflatoxin B1 (0-1 μg/mL; 24-72 h) 剂量和时间依赖性地抑制分化和未分化的 Caco-2 细胞的生长，增加细胞中 DNA 的损伤和 ROS 的积累。Aflatoxin B1 (0-200 mg/L; 48 h) 抑制 HEK 293 细胞活力并诱导细胞凋亡 (apoptosis)。Cell Cytotoxicity Assay Cell Line:Differentiated and undifferentiated Caco-2 cells Concentration:0, 0.01, 0.05, 0.1, 0.5 and 1 μg/mL Incubation Time:24, 48 and 72 h Result:Cytotoxicity was dose-dependent at doses of 0.05-1 μg/mL, and increased cell death in both differentiated and undifferentiated cells at concentrations of 0.5 μg/mL and above.Cell Cytotoxicity Assay Cell Line:HEK 293 cells Concentration:0, 5, 10, 50, 100 and 200 mg/L Incubation Time:48 h Result:Upregulated the expression levels of proline dehydrogenase (PRODH) and pro-apoptotic factors (Bax, Caspase-3), and downregulated the expression of apoptosis inhibitor Bcl-2.

黄曲霉毒素B1

Proteasome/Ubiquitin

Endogenous Metabolite

Human Endogenous Metabolite

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1162-65-8

312.281

C17H12O6

>98% (HPLC)

DMF : 33.33 mg/mL (106.73 mM) DMSO : 30 mg/mL (96.07 mM)

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(-20℃)

With Ice Pack

≥ 2 years