Deoxynivalenol

本公司产品仅用于科研实验，不得用于人体或动物的临床与诊断

Deoxynivalenol是单端孢菌素家族 (trichothecenes family) 中一种具有口服活性的真菌毒素，通过紧密连接处的细胞旁路来穿过肠粘膜。Deoxynivalenol 转运不受P-糖蛋白 (PgP) 或多药耐药相关蛋白 (MRPs) 抑制剂的影响。

Deoxynivalenol, an orally active mycotoxin of the trichothecenes family, crosses the intestinal mucosa by a paracellular pathway through the tight junctions. The Deoxynivalenol transport is not affected by P-glycoprotein (PgP) or multidrug resistance-associated proteins (MRPs) inhibitors.

Cell Viability Assay Cell Line:IPEC-1 and IPEC-J2 cells Concentration:100-4000 ng/mL Incubation Time: 24-72 h Result:Reduced the cell count in a time and dose dependent.Increased LDH signals in IPEC-1 after 48 h and 72 h incubation with 2000 ng/mL and in IPEC-J2 after 48 h incubation with 2000 ng/mL.Reduced NR uptake in IPEC-1 and IPEC-J2 cells at the doses of 2000 ng/mL.Decreased the MTT signal in IPEC-1 to 60% and 40% of the control after 48 h and 72 h at the doses of 2000 ng/mL.Decreased the MTT signal in IPEC-J2 to 70% and 58% of the control after 48 h and 72 h at the doses of 2000 ng/mL.Cell Proliferation Assay Cell Line:Caco-2 cells Concentration:0-10 μg/mL Incubation Time:48 h Result:Inhibited the proliferation of cells in a dose-dependent manner.Cell Cycle Analysis Cell Line:IPEC-1 and IPEC-J2 cells Concentration:200-2000 ng/mL Incubation Time:24-72 h Result:Increased percentages of pre-G1, S and G2/M phases and decreased of the G0/G1 phase in response to 2000 ng/mL after 48 h in IPEC-1 cells.Decreased the G0/G1 phase in IPEC-J2 cell at the doses of 2000 ng/mL after 48 h.Increased the percentage of pre-G1 in IPEC-1 cells after 72 h.Increased the G2/M phase in IPEC-1 and IPEC-J2 cells at the doses of 2000 ng/mL after 72 h.Apoptosis Analysis Cell Line:IPEC-1 and IPEC-J2 cells Concentration:200-2000 ng/mL Incubation Time:6-72 h Result:Increased the caspase 3 activity after 6 h, 8 h, 48 h and 72 h of incubation with 2000 ng/mL.Reduced the caspase 3 activity to the control level after 24 h of incubation with 2000 ng/mL.Western Blot Analysis Cell Line:Caco-2 cells Concentration:2 μg/mL Incubation Time:5-240 min Result:Increased the phosphorylation of Erk1/Erk2 during 5 min.Increased the phosphorylation of p38 from 5 to 30 min.Western Blot Analysis Cell Line: IPEC-1 and IPEC-J2 cells Concentration: 200-2000 ng/mL Incubation Time:48 h Result:Reduced the protein level of ZO-1 at the doses of 2000 ng/mL.

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Others

Other Targets

Others

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51481-10-8

296.32

C15H20O6

>98% (HPLC)

In Vitro:?Ethanol 中的溶解度 : 30 mg/mL (101.24 mM; 超声加热助溶) DMSO 中的溶解度 : 25 mg/mL (84.37 mM; 超声加热助溶）

CC1=C[C@H]2O[C@@H]3[C@H](O)C[C@@](C)([C@]33CO3)[C@@]2(CO)[C@H](O)C1=O

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(-20℃)

With Ice Pack

≥ 2 years