Demeclocycline

本公司产品仅用于科研实验，不得用于人体或动物的临床与诊断

Demeclocycline 是一种具有口服活性的四环素类抗生素，通过与 30S 核糖体亚单位结合来抑制氨基酰 tRNA 的结合，从而影响蛋白质的合成。Demeclocycline 对广谱的细菌感染表现出抗菌活性。

Demeclocycline is an orally active tetracycline antibiotic. Demeclocycline impairs protein synthesis by binding to the 30S ribosomal subunit to inhibit binding of aminoacyl tRNA. Demeclocycline shows anti-bacterial activitise to a wide variety of bacterial infections.

Demeclocycline (0-100 μM; 24 h) treatment reduces AQP2 abundance in mpkCCD cells.Demeclocycline (10 μM; 24 h) treatment promotes the activity of monocytes and macrophages.Demeclocycline (1-10 μM; 72 h) treatment directly affects the growth of brain tumorinitiating cells.Western Blot Analysis Cell Line:MpkCCD cells Concentration:0-100 μM Incubation Time:24 hours Result:Decreased AQP2 abundance in mpkCCD cells, with significant effects at 50 μM.Cell Viability Assay Cell Line:mouse bone marrow derived macrophages and monocytes Concentration:10 μM Incubation Time:24 hours Result:Enhanced TNF-α production and modulated monocyte functions.Cell Viability Assay Cell Line:brain tumorinitiating cells Concentration:1, 5, and 10 μM Incubation Time:72 hours Result:Inhibited cells growth in two ways: using monocytes as an intermediary, and directly by affecting the proliferation and sphere-forming capacity of brain tumorinitiating cells.

Demeclocycline (Intraperitoneal injection; 40 mg/kg; once daily; 48 h) treatment results in a significant reduction of hyponatremia and a significant correction of the hypoosmolality, and is not nephrotoxic.Animal Model:Male Wistar rats induced with hyponatremia Dosage:40 mg/kg Administration:Intraperitoneal injection; 40 mg/kg; once daily; 48 hours Result:Increased urine volume, decreased urine osmolality, and caused a significantly increased fractional excretion of water.Animal Model:Male Wistar rats induced with hyponatremiaDosage:40 mg/kg Administration:Intraperitoneal injection; 40 mg/kg; once daily; 48 hours Result:Indicated the effect in the renal inner medulla for AQP2 and AC5/6 specifically, and not secondary toxicity effect.

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GPCR/G Protein

Antibacterial

Antibacterial

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127-33-3

464.85

C21H21ClN2O8

>98% (HPLC)

In Vitro:?H2O 中的溶解度 : 40 mg/mL (86.05 mM; 超声助溶)DMSO 中的溶解度 : 25 mg/mL (53.78 mM; 超声助溶 )

[H][C@]12C[C@@]3([H])[C@H](N(C)C)C(O)=C(C(N)=O)C(=O)[C@@]3(O)C(O)=C1C(=O)c1c(O)ccc(Cl)c1[C@H]2O

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(-20℃)

With Ice Pack

≥ 2 years