m-3M3FBS

本公司产品仅用于科研实验，不得用于人体或动物的临床与诊断

m-3M3FBS 是一种磷脂酶 C (PLC) 激活剂。 m-3M3FBS 刺激中性粒细胞中短暂的细胞内钙浓度 ([Ca(2+)](i)) 增加。

m-3M3FBS is a phospholipase C (PLC) activator. m-3M3FBS stimulated a transient intracellular calcium concentration ([Ca(2+)](i)) increase in neutrophils.?Moreover, m-3M3FBS stimulated the formation of inositol phosphates in U937 cells, indicating that it stimulates PLC activity.?The compound showed no cell-type specificity in terms of [Ca(2+)](i) increase in the various cell lines including leukocytes, fibroblasts, and neuronal cells,?also ruled out the possible involvement of heterotrimeric G proteins in m-3M3FBS-stimulated signaling by confirming the following: 1) pertussis toxin does not inhibit m-3M3FBS-induced [Ca(2+)](i) increase;?2) m-3M3FBS does not stimulate cyclic AMP generation;?and 3) the inhibition of G(q) by the regulator of G protein-signaling 2 does not affect the m-3M3FBS-induced [Ca(2+)](i) increase.?m-3M3FBS stimulated PLC activity in vitro.?The purified isoforms of PLC that were tested (i.e., beta2, beta3, gamma1, gamma2, and delta1) were activated by m-3M3FBS and showed no isoform specificity.

m-3M3FBS (5-50 μM) stimulates the formation of inositol phosphates in U937 cells.m-3M3FBS (50 μM; 24 hours) inhibits the growth of the leukemic cell lines U937 and THP-1, but not primary monocytes.m-3M3FBS (50 μM; 24 hours) induces U937 cell apoptosis. Cell Viability AssayCell Line:U937 and THP-1 cells Concentration:50 μM Incubation Time:24 hours Result:Inhibited the growth of the leukemic cell lines U937 and THP-1, but not primary monocytes.Cell Viability Assay Cell Line:U937 cells Concentration:50 μM Incubation Time:24 hoursResult:The apoptotic rate of m-3M3FBS-treated cells was 53.9%.

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Metabolic Enzyme/Protease

Phosphatase

PLC|Apoptosis

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200933-14-8

343.36

C16H16F3NO2S

>98% (HPLC)

In Vitro:?DMSO : 100 mg/mL (291.24 mM)

Cc1cc(C)c(c(C)c1)S(=O)(=O)Nc1cccc(c1)C(F)(F)F

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(-20℃)

With Ice Pack

≥ 2 years