Nifurtimox

本公司产品仅用于科研实验，不得用于人体或动物的临床与诊断

Nifurtimox 是一种抗原虫剂（对克氏锥虫上鞭毛体的 Taluahuén、LQ 和 Brener 菌株的 IC50 值分别为 9.91、12.28 和 10.44 μM）。

Nifurtimox is an antiprotozoal agent (IC50s = 9.91 12.28 and 10.44 μM against Taluahuén LQ and Brener strains of T. cruzi- epimastigotes respectively).(In Vitro):Nifurtimox affects enzyme activity of lactate dehydrogenase (LDH). To differentiate if this effect is a result of a reduced LDH activity or a shift in pyruvate metabolism due to activation of PDH, the enzyme activity of LDH is determined after 4 h treatment with 50 μg/mL Nifurtimox. Compared to the untreated control, the LDH activity is significantly reduced for LA-N-1 (P=0.005), IMR-32 (P=0.009), LS (P=0.0035) and SK-N-SH (P=0.0065). Nifurtimox reduces cell viability and induces cell cycle arrest and apoptosis in neuroblastoma cells. To characterize the cytotoxic impacts of Nifurtimox on neuroblastoma, 4 cell lines are subjected to several experiments. Cell viability is reduced for all 4 neuroblastoma cell lines after 24 h incubation with 50 μg/mL to an average of 66%, 63%, 62% and 75% (LA-N-1, IMR-32 LS and SK-N-SH, respectively). The reduction is significant compared to the untreated control (P<0.01) and the vehicle control with DMSO (P<0.05) for all cell lines.

Nifurtimox affects enzyme activity of lactate dehydrogenase (LDH). To differentiate if this effect is a result of a reduced LDH activity or a shift in pyruvate metabolism due to activation of PDH, the enzyme activity of LDH is determined after 4 h treatment with 50 μg/mL Nifurtimox. Compared to the untreated control, the LDH activity is significantly reduced for LA-N-1 (P=0.005), IMR-32 (P=0.009), LS (P=0.0035) and SK-N-SH (P=0.0065). Nifurtimox reduces cell viability and induces cell cycle arrest and apoptosis in neuroblastoma cells. To characterize the cytotoxic impacts of Nifurtimox on neuroblastoma, 4 cell lines are subjected to several experiments. Cell viability is reduced for all 4 neuroblastoma cell lines after 24 h incubation with 50 μg/mL to an average of 66%, 63%, 62% and 75% (LA-N-1, IMR-32 LS and SK-N-SH, respectively). The reduction is significant compared to the untreated control (P<0.01) and the vehicle control with DMSO (P<0.05) for all cell lines.

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BAY-2502 | BAY-A-2502

Microbiology/Virology

Parasite

Parasite

Cancer

Medulloblastoma; Neuroblastoma

23256-30-6

287.3

C10H13N3O5S

>98% (HPLC)

DMSO: 50 mg/mL (174.03 mM);Water: Insoluble

CC1CS(=O)(=O)CCN1\N=C\c1ccc(o1)[N+]([O-])=O

(E)-3-methyl-4-(((5-nitrofuran-2-yl)methylene)amino)thiomorpholine 11-dioxide

(-20℃)

With Ice Pack

≥ 2 years