Gemcitabine HCl

本公司产品仅用于科研实验，不得用于人体或动物的临床与诊断

Gemcitabine HCl 是一种 DNA 合成抑制剂，在 PANC1、MIAPaCa2、BxPC3 和 Capan2 细胞中的 IC50 分别为 50 nM、40 nM、18 nM 和 12 nM。

Gemcitabine HCl is a DNA synthesis inhibitor with IC50 of 50 nM, 40 nM, 18 nM and 12 nM in PANC1, MIAPaCa2, BxPC3 and Capan2 cells, respectively.(In Vitro):Gemcitabine Hydrochloride (purchased from MedChem Express, 0.003-1 μM; 3 days) kills both mouse and human senescent cells effectively and potently.Gemcitabine Hydrochloride inhibits the growth of BxPC-3, Mia Paca-2, PANC-1, PL-45 and AsPC-1 cells with IC50s of 37.6, 42.9, 92.7, 89.3 and 131.4 nM, respectively. (In Vivo):Gemcitabine Hydrochloride can be administered via endotracheal spray in rats without marked toxicity with a maximum tolerated dose of 4 mg/kg once a week for 9 weeks. The toxicity of Gemcitabine is lower via lung than oral administration at dosages of 2, 4, and 6 mg/kg.Treatment of the LSL-KrasG12D/+; LSL-Trp53R172H; Pdx-1-Cre mice with either Gemcitabine (50 mg/kg, i.p.) or the combination DMAPT/Gemcitabine Hydrochloride significantly increases the median survival time by more than 30 days compared to the placebo group (254.5 or 255 days vs. 217.5 days, respectively).

Gemcitabine Hydrochloride (purchased from MedChem Express, 0.003-1 μM; 3 days) kills both mouse and human senescent cells effectively and potently. Gemcitabine Hydrochloride inhibits the growth of BxPC-3, Mia Paca-2, PANC-1, PL-45 and AsPC-1 cells with IC50s of 37.6, 42.9, 92.7, 89.3 and 131.4 nM, respectively. Cell Viability Assay Cell Line:Non-senescent and replication-induced senescent new born dermal fibroblasts (NBFs)Concentration:0.003, 0.01, 0.03, 0.1, 0.3, 1 μM Incubation Time:3 days Result:Killed replication-induced senescent NBFs for 3 days with 11.0% cell viability.

Gemcitabine Hydrochloride can be administered via endotracheal spray in rats without marked toxicity with a maximum tolerated dose of 4 mg/kg once a week for 9 weeks. The toxicity of Gemcitabine is lower via lung than oral administration at dosages of 2, 4, and 6 mg/kg.Treatment of the LSL-KrasG12D/+; LSL-Trp53R172H; Pdx-1-Cre mice with either Gemcitabine (50 mg/kg, i.p.) or the combination DMAPT/Gemcitabine Hydrochloride significantly increases the median survival time by more than 30 days compared to the placebo group (254.5 or 255 days vs. 217.5 days, respectively).

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Cell Cycle/DNA Damage

DNA/RNA Synthesis

DNA synthesis (BxPC3 cells)| DNA synthesis (Capan2 cells)| DNA synthesis (MIAPaCa2 cells)| DNA synthesis (PANC1 cells)

Cancer

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122111-03-9

299.66

C9H11F2N3O4.HCI

>98% (HPLC)

DMSO:<1 mg/mL (<1 mM); Ethanol:<1 mg/mL (<1 mM); Water：19 mg/mL (63.4 mM)

Cl.NC1=NC(=O)N(C=C1)[C@@H]1O[C@H](CO)[C@@H](O)C1(F)F |r,c:6,t:1|

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(-20℃)

With Ice Pack

≥ 2 years