Robotnikinin

本公司产品仅用于科研实验，不得用于人体或动物的临床与诊断

Robotnikinin 是一种小分子，可结合细胞外 Sonic Hedgehog (Shh) 蛋白 (Kd=3.1 uM) 并阻断细胞系中的 Shh 信号传导。

Robotnikinin is asmall molecule that binds the extracellular Sonic Hedgehog (Shh) protein (Kd=3.1 uM) and blocks Shh-signaling in cell lines, human primary keratinocytes and synthetic model of human skin; displays significant repression of Shh-induced Gli1/Gli2, and interferes with the ability of the ShhN protein complex to relay its signal efficiently to Ptc1.

Robotnikinin demonstrates ShhN-binding capacity at concentrations between 1.56 μM and 25 μM, with a KD of 3.1 μM derived from kinetic data.Robotnikinin (50 μM) prevents the ShhN-induced LC3-II increase.Robotnikinin (5 μM) downregulates classical NF-κB pathway proteins in H929 and U266 cell lines cocultured with HS-5 cells, suggesting a link between Hh signaling and the NF-κB pathway in MM. RT-PCR Cell Line:NCI-H929 or U266 cells.Concentration:5 μM.Incubation Time:48 h.Result:Downregulated classical NF-κB pathway proteins in H929 and U266 cell lines cocultured with HS-5 cells.

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Robotnikinin

GPCR/G Protein

Hedgehog (Hh)

Hedgehog (Hh)

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1132653-79-2

454.951

C25H27ClN2O4

>98% (HPLC)

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O=C(NCC1=CC=C(Cl)C=C1)C[C@H](C/C=C/CC2)C(NC[C@@H](C3=CC=CC=C3)OC2=O)=O

N-[(4-chlorophenyl)methyl]-5,12-dioxo-2R-phenyl-1-oxa-4-azacyclododec-8E-ene-6S-acetamide

(-20℃)

With Ice Pack

≥ 2 years