Calcipotriol

本公司产品仅用于科研实验，不得用于人体或动物的临床与诊断

卡泊三醇是一种合成的 VitD3 类似物。对维生素 D 受体具有高亲和力。当 NHEK 细胞未用 IL-17A 或 IL-22 刺激时，卡泊三醇会轻微增强 (0.2 nM) IL-8 mRNA 表达，或没有影响 (2-20 nM)。

Calcipotriol is a synthetic VitD3 analogue. Which has a high affinity for the vitamin D receptor.Calcipotriol slightly enhances (0.2 nM) IL-8 mRNA expression or has no effect (2-20 nM), when NHEK cells are not stimulated with IL-17A or IL-22. The addition of IL-17A and IL-22 markedly increased the mRNA expression of IL-8. This enhanced IL-8 mRNA expression is suppressed by Calcipotriol at 2, 20 and 40 nM in a dose dependent manner. Treatment of natural killer (NK) cells with drugs modulates their expression of NK cytotoxicity receptors or KIR. Human NK cells are pre-treated with 100, 10 or 1 ng/mL of 1,25(OH)2D3, Calcipotriol or FTY720 for 4 h. All three concentrations of Calcipotriol, 1,25(OH)2D3 and FTY720 significantly up-regulate the expression of NKp30 on the surface of NK cells after 4 h incubation.Compare with placebo (linear regression model), the weight gain is significantly smaller in the groups treated with Diclofenac plus Calcipotriol (p=0.018) and Diclofenac plus DFMO plus Calcipotriol (p=0.002). Survival is equally distributed between the groups. One out of the 32 animals in each of the groups has died, except for the Diclofenac plus DFMO plus Calcipotriol group, where all animals survived.(In Vitro):When NHEK cells are not stimulated with IL-17A or IL-22, Calcipotriol slightly enhances (0.2 nM) IL-8 mRNA expression or has no effect (2-20 nM). The addition of IL-17A and IL-22 markedly increased the mRNA expression of IL-8, confirming our previous study. This enhanced IL-8 mRNA expression is suppressed by Calcipotriol at 2, 20 and 40 nM in a dose dependent manner. Treatment of natural killer (NK) cells with drugs modulates their expression of NK cytotoxicity receptors or KIR. Human NK cells are pre-treated with 100, 10 or 1 ng/mL of 1,25(OH)2D3, Calcipotriol or FTY720 for 4 h. All three concentrations of 1,25(OH)2D3, Calcipotriol and FTY720 significantly up-regulate the expression of NKp30 on the surface of NK cells after 4 h incubation.(In Vivo):One out of the 32 animals in each of the groups has died, except for the Diclofenac plus DFMO plus Calcipotriol group, where all animals survived. Survival is equally distributed between the groups. The weight gain is significantly smaller in the groups treated with Diclofenac plus Calcipotriol (p=0.018) and Diclofenac plus DFMO plus Calcipotriol (p=0.002) compare with placebo (linear regression model).

When NHEK cells are not stimulated with IL-17A or IL-22, Calcipotriol slightly enhances (0.2 nM) IL-8 mRNA expression or has no effect (2-20 nM). The addition of IL-17A and IL-22 markedly increased the mRNA expression of IL-8, confirming our previous study. This enhanced IL-8 mRNA expression is suppressed by Calcipotriol at 2, 20 and 40 nM in a dose dependent manner. Treatment of natural killer (NK) cells with drugs modulates their expression of NK cytotoxicity receptors or KIR. Human NK cells are pre-treated with 100, 10 or 1 ng/mL of 1,25(OH)2D3, Calcipotriol or FTY720 for 4 h. All three concentrations of 1,25(OH)2D3, Calcipotriol and FTY720 significantly up-regulate the expression of NKp30 on the surface of NK cells after 4 h incubation.

One out of the 32 animals in each of the groups has died, except for the Diclofenac plus DFMO plus Calcipotriol group, where all animals survived. Survival is equally distributed between the groups. The weight gain is significantly smaller in the groups treated with Diclofenac plus Calcipotriol (p=0.018) and Diclofenac plus DFMO plus Calcipotriol (p=0.002) compare with placebo (linear regression model).

MC 903,Calcipotriene

Metabolic Enzyme/Protease

Vitamin

Vitamin D receptor

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112965-21-6

412.6

C27H40O3

>98% (HPLC)

DMSO:100 mg/mL (242.37 mM);Ethanol:50 mg/mL (121.18 mM; Need ultrasonic)

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(-20℃)

With Ice Pack

≥ 2 years